Training: RNA-Seq Analysis

Sequencing of RNA molecules allows investigation of various aspects of biology, including changes in gene expression, post-translational modifications and identification of novel transcripts. This module focusses on the most common usage of RNA-Seq: determination of differential gene expression. Amongst the topics covered are considerations in experimental design, sequencing strategy, assessment of RNA-Seq data, determination of differential expression and visualisation of results.

After completing this module, participants will be able to:

• Design reproducible RNA-Seq experiments
• Understand the importance of replicates and sample variance
• Determine differential gene expression from read alignments
• Analyse RNA-Seq data using pseudo-alignment methods
• Produce visualisations of RNA-Seq results

• Introduction to Linux
• The HPC Cluster
• Introduction to NGS
• Read Alignment
• Introductory Statistics with R

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