Training: ChIP-Seq Analysis

ChIP-Seq allows the investigation of proteins interacting with DNA though the immunoprecipitation of bound proteins, producing a library of DNA molecules bound to the protein of interest. Sequencing of these fragments reveals the location of the binding sites within the genome. This module will discuss ChIP-Seq experimental design, identification of peaks in read alignments indicating binding loci and differential peak calling between experimental conditions.

After completing this module, participants will be able to:

• Design reproducible ChIP-Seq experiments
• Understand the different type of signals resulting from different kinds of binding interaction
• Identify peaks in read alignments resulting from protein binding
• Identify differential binding between experimental conditions

• Introduction to Linux
• The HPC Cluster
• Introduction to NGS
• Read Alignment

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