Module Overview

ChIP-Seq allows the investigation of proteins interacting with DNA though the immunoprecipitation of bound proteins, producing a library of DNA molecules bound to the protein of interest. Sequencing of these fragments reveals the location of the binding sites within the genome. This module will discuss ChIP-Seq experimental design, identification of peaks in read alignments indicating binding loci and differential peak calling between experimental conditions.

Learning Outcomes

After completing this module, participants will be able to:
  • Design reproducible ChIP-Seq experiments
  • Understand the different type of signals resulting from different kinds of binding interaction
  • Identify peaks in read alignments resulting from protein binding
  • Identify differential binding between experimental conditions

Prerequisite Modules/Knowledge

  • Introduction to Linux
  • The HPC Cluster
  • Introduction to NGS
  • Read Alignment

Course Schedule

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