FastQCFastQC Report
Wed 26 May 2021
DRR274673_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR274673_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278944
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTT9650.34594757370655044No Hit
GTCCAACTTAGGGTCAATTTCTGTACAAACAACACCATCCAATTTATAAG9190.32945680853504644No Hit
GTGAAATTGGGCCTCATAGCACATTGGTAAACACCAGATGGTGAACCATT8020.2875129058162212No Hit
GCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTC7570.27138063553974995No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7310.2620597682688998No Hit
ATCAGATTCAACTTGCATGGCATTGTTAGTAGCCTTATTTAAGGCTCCTG6580.23588964093151313No Hit
GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTC6380.22871974303085926No Hit
GCAGCATCTACAGCAAAAGCACAGAAAGATAATACAGTTGAATTGGCAGG6290.225493288975565No Hit
CTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAG6290.225493288975565No Hit
AAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGA6170.22119135023517264No Hit
GTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAG6140.22011586555007456No Hit
GTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCT6030.21617242170471493No Hit
GTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATA5980.21437994722955145No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA5950.21330446254445334No Hit
GTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGTCCAA5860.2100780084891591No Hit
GGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAG5610.20111563611334174No Hit
GTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTG5440.19502122289778595No Hit
GCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG5410.19394573821268785No Hit
GCCATCTTTAAGATGTTGACGTGCCTCTGATAAGACCTCCTCCACGGAGT5390.19322874842262247No Hit
GTTGAACCTTTCTACAAGCCGCATTAATCTTCAGTTCATCACCAATTATA5310.1903607892623609No Hit
GGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTG5110.18319089136170702No Hit
GCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTG5070.18175691178157624No Hit
GGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTT4950.1774549730411839No Hit
GGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCT4900.1756624985660204No Hit
GTGAAGTTCTTTTCTTGTGCAGGGACATAAGTCACATGCAAGAAGACTAC4870.17458701388092232No Hit
GCACAGGTGACAATTTGTCCACCGACAATTTCACAAGCACAGGTTGAGAT4680.16777561087530116No Hit
GCCAGAGATGTCACCTAAATCAACATCTGGTGATGTATGATTCTTAAAAT4630.16598313640013768No Hit
ATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTATTATGTTCAAGGG4610.16526614661007227No Hit
CTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCA4600.16490765171503957No Hit
CTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGC4580.16419066192497417No Hit
CAAGCACCAAGGTCACGGGGTGTCATGTTTTCAACTTTGTTATAGGTGAG4450.15953022828954916No Hit
GTTCACCTAAGTTGGCGTATACGCGTAATATATCTGGGTTTTCTACAAAA4430.15881323849948376No Hit
GGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTT4420.15845474360445108No Hit
GTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTT4370.1566622691292876No Hit
GCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCA4360.1563037742342549No Hit
GGAAAAGAAAGGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATC4250.15236033038889527No Hit
ATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAA4190.15020936101869908No Hit
GTACTCTATAAGTTTTGATGGTGTGTAACAGATGTTACCAACTGCACTAA4180.14985086612366638No Hit
GTCCACACTCTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAG4180.14985086612366638No Hit
ATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAA4160.149133876333601No Hit
ATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAG4160.149133876333601No Hit
CTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTT4070.14590742227830675No Hit
ATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCC4060.14554892738327407No Hit
GTGGCTTTATTAGTTGCATTGTTAACATGCCAAACAATAGGTTTATGTAA4020.1441149478031433No Hit
GCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGC3990.14303946311804522No Hit
GTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCA3830.13730354479752208No Hit
GGACAGTAAACTACGTCATCAAGCCAAAGACCGTTAAGTGTAGTTGTACC3820.13694504990248937No Hit
CAAAAACTTGTCCATTAGCACACAATGGAAAACTAATGGGTGGTTTATGT3810.1365865550074567No Hit
CGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCAT3810.1365865550074567No Hit
GTCCTGTTACATTTTCAGCTTGTAAAGTTGCCACATTCCTACGTGGAATT3630.1301336468968682No Hit
ATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTC3600.1290581622117701No Hit
ATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTA3550.12726568773660663No Hit
GGTGAATTGCCCTCGTATGTTCCAGAAGAGCAAGGTTCTTTTAAAAGTAC3550.12726568773660663No Hit
ATAAACACCAAGAGTCAGTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGC3530.12654869794654122No Hit
CGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATG3490.12511471836641044No Hit
ATTCTTTGCTATAGTAGTCGGCATAGATGCTTTAATTCTAGAATTTGTAC3490.12511471836641044No Hit
GCGGCCTTCTGTAAAACACGCACAGAATTTTGAGCAGTTTCAAGAGTGCG3480.12475622347137777No Hit
CGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGA3400.12188826431111621No Hit
GTCAGAATAGTGCCATGGAGTGGCACGTTGAGAAGAATGTTAGTTTCTGG3400.12188826431111621No Hit
AATTGAGCAGGGTCGCCAATGTACACATAGTGCTTAGCACGTAATCTGGC3390.12152976941608351No Hit
CTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTG3360.12045428473098543No Hit
CTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTG3340.11973729494092004No Hit
GTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCA3230.11579385109556041No Hit
GGTCAGAATAGTGCCATGGAGTGGCACGTTGAGAAGAATGTTAGTTTCTG3230.11579385109556041No Hit
GGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTA3200.1147183664104623No Hit
CAAACTGCCACCATCACAACCAGGCAAGTTAAGGTTAGATAGCACTCTAG3160.11328438683033154No Hit
CAGCTGTCCAACCTGAAGAAGAATCACCAGGAGTCAAATAACTTCTATGT3110.11149191235516806No Hit
GCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCT3110.11149191235516806No Hit
GTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTACTGTACAA3100.11113341746013536No Hit
GTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTAT3100.11113341746013536No Hit
CTTTTCTCCAAGCAGGGTTACGTGTAAGGAATTCTCTTACCACGCCTATT3090.11077492256510268No Hit
GCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATCATTAGTAAG3080.11041642767006998No Hit
CTTTCCACATACCGCAGACGGTACAGACTGTGTTTTTAAGTGTAAAACCC3080.11041642767006998No Hit
GCCAAATTGTTGGAAAGGCAGAAACTTTTTGTTAGACTCAGTAAGAACAC3050.1093409429849719No Hit
GGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAAC2980.10683147871974304No Hit
AACATGTTGTGCCAACCACCATAGAATTTGCTTGTTCCAATTACTACAGT2930.10503900424457956No Hit
GGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTG2900.10396351955948148No Hit
CCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAA2900.10396351955948148No Hit
CACCAACCTTAGAATCAAGATTGTTAGAATTCCAAGCTATAACGCAGCCT2870.1028880348743834No Hit
CTAGGTTCCATTGTTCAAGGAGCTTTTTAAGCTCTTCAACGGTAATAGTA2860.1025295399793507No Hit
GTTCCTGGCAATTAATTGTAAAAGGTGAACAGGAAACTGTATAATTACCG2850.102171045084318No Hit
GGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCA2830.10145405529425261No Hit
GTACCAACAGCTTCTCTAGTAGCATGACACCCCTCGACATCGAAGCCAAT2800.10037857060915453No Hit
GTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATA2790.10002007571412183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)