FastQCFastQC Report
Wed 26 May 2021
DRR274673_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR274673_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278944
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTT9430.3380606860158311No Hit
GTCCAACTTAGGGTCAATTTCTGTACAAACAACACCATCCAATTTATAAG9010.323003900424458No Hit
ATCAGATTCAACTTGCATGGCATTGTTAGTAGCCTTATTTAAGGCTCCTG8560.3068716301479867No Hit
GTGAAATTGGGCCTCATAGCACATTGGTAAACACCAGATGGTGAACCATT7970.2857204313410577No Hit
GCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTC7720.2767580589652403No Hit
GCAGCATCTACAGCAAAAGCACAGAAAGATAATACAGTTGAATTGGCAGG7320.2624182631639326No Hit
CTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAG6830.2448520133073305No Hit
GTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCT6510.23338017666628424No Hit
AAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGA6430.23051221750602274No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA6150.22047436044510724No Hit
GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTC6020.21581392680968225No Hit
GTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAG5980.21437994722955145No Hit
GTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATA5900.21151198806928986No Hit
GCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG5820.20864402890902836No Hit
GTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGTCCAA5580.20004015142824366No Hit
GTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTG5550.19896466674314559No Hit
GGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAG5290.1896437994722955No Hit
GCCATCTTTAAGATGTTGACGTGCCTCTGATAAGACCTCCTCCACGGAGT5170.18534186073190317No Hit
CTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCA5130.1839078811517724No Hit
GGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTG5100.18283239646667432No Hit
GTTGAACCTTTCTACAAGCCGCATTAATCTTCAGTTCATCACCAATTATA5010.17960594241138006No Hit
GCCAGAGATGTCACCTAAATCAACATCTGGTGATGTATGATTCTTAAAAT5000.17924744751634739No Hit
ATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTATTATGTTCAAGGG4970.17817196283124928No Hit
GGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCT4890.17530400367098772No Hit
ATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAA4850.17387002409085694No Hit
GCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTG4820.17279453940575887No Hit
ATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAG4700.16849260066536653No Hit
GCACAGGTGACAATTTGTCCACCGACAATTTCACAAGCACAGGTTGAGAT4670.16741711598026845No Hit
CTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGC4620.16562464150510497No Hit
CAAGCACCAAGGTCACGGGGTGTCATGTTTTCAACTTTGTTATAGGTGAG4570.1638321670299415No Hit
ATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAA4520.16203969255477801No Hit
CTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTT4520.16203969255477801No Hit
GTGAAGTTCTTTTCTTGTGCAGGGACATAAGTCACATGCAAGAAGACTAC4490.16096420786967994No Hit
GGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTT4490.16096420786967994No Hit
GGAAAAGAAAGGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATC4330.15522828954915682No Hit
GTTCACCTAAGTTGGCGTATACGCGTAATATATCTGGGTTTTCTACAAAA4300.15415280486405875No Hit
GCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCA4280.15343581507399334No Hit
GTCCACACTCTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAG4250.15236033038889527No Hit
ATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTC4170.1494923712286337No Hit
GTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTT4120.14769989675347023No Hit
GGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTT4060.14554892738327407No Hit
GCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGC4050.14519043248824137No Hit
ATAAACACCAAGAGTCAGTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGC4000.1433979580130779No Hit
GTCAGAATAGTGCCATGGAGTGGCACGTTGAGAAGAATGTTAGTTTCTGG3870.13873752437765285No Hit
ATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCC3820.13694504990248937No Hit
GTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCA3810.1365865550074567No Hit
GTACTCTATAAGTTTTGATGGTGTGTAACAGATGTTACCAACTGCACTAA3800.136228060112424No Hit
AATTGAGCAGGGTCGCCAATGTACACATAGTGCTTAGCACGTAATCTGGC3800.136228060112424No Hit
GTGGCTTTATTAGTTGCATTGTTAACATGCCAAACAATAGGTTTATGTAA3770.13515257542732592No Hit
CTTTTCTCCAAGCAGGGTTACGTGTAAGGAATTCTCTTACCACGCCTATT3660.1312091315819663No Hit
ATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTA3650.13085063668693359No Hit
GCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATCATTAGTAAG3640.13049214179190088No Hit
CAAACTGCCACCATCACAACCAGGCAAGTTAAGGTTAGATAGCACTCTAG3610.1294166571068028No Hit
GCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCT3600.1290581622117701No Hit
CTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTG3590.1286996673167374No Hit
ATTCTTTGCTATAGTAGTCGGCATAGATGCTTTAATTCTAGAATTTGTAC3590.1286996673167374No Hit
CGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATG3570.12798267752667203No Hit
GCGGCCTTCTGTAAAACACGCACAGAATTTTGAGCAGTTTCAAGAGTGCG3560.12762418263163933No Hit
GTCCTGTTACATTTTCAGCTTGTAAAGTTGCCACATTCCTACGTGGAATT3510.12583170815647585No Hit
CTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTG3510.12583170815647585No Hit
CGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGA3460.12403923368131237No Hit
GTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTAT3420.12260525410118159No Hit
GGACAGTAAACTACGTCATCAAGCCAAAGACCGTTAAGTGTAGTTGTACC3410.12224675920614891No Hit
CTTTCCACATACCGCAGACGGTACAGACTGTGTTTTTAAGTGTAAAACCC3360.12045428473098543No Hit
CTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTC3350.12009578983595273No Hit
GGTGAATTGCCCTCGTATGTTCCAGAAGAGCAAGGTTCTTTTAAAAGTAC3350.12009578983595273No Hit
GTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTACTGTACAA3340.11973729494092004No Hit
CAGCTGTCCAACCTGAAGAAGAATCACCAGGAGTCAAATAACTTCTATGT3340.11973729494092004No Hit
GGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTA3310.11866181025582195No Hit
CGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCAT3300.11830331536078927No Hit
CAAAAACTTGTCCATTAGCACACAATGGAAAACTAATGGGTGGTTTATGT3250.11651084088562579No Hit
GCCAAATTGTTGGAAAGGCAGAAACTTTTTGTTAGACTCAGTAAGAACAC3210.115076861305495No Hit
GGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCA3210.115076861305495No Hit
GCTTCAACAGCTTCACTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGT3160.11328438683033154No Hit
GTACCAACAGCTTCTCTAGTAGCATGACACCCCTCGACATCGAAGCCAAT3140.11256739704026614No Hit
CCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAA3090.11077492256510268No Hit
CACCAACCTTAGAATCAAGATTGTTAGAATTCCAAGCTATAACGCAGCCT3060.10969943788000458No Hit
CCTACCTCCCTTTGTTGTGTTGTAGTAAGCTAACGCATTGTCATCAGTGC3050.1093409429849719No Hit
GGTCAGAATAGTGCCATGGAGTGGCACGTTGAGAAGAATGTTAGTTTCTG3030.1086239531949065No Hit
GGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTG2930.10503900424457956No Hit
GTTCCTGGCAATTAATTGTAAAAGGTGAACAGGAAACTGTATAATTACCG2890.10360502466444878No Hit
AATACAAGCACCAAGGTCACGGGGTGTCATGTTTTCAACTTTGTTATAGG2870.1028880348743834No Hit
CGATGACTTGGTTAGCATTAATACAGCCACCATCGTAACAATCAAAGTAC2860.1025295399793507No Hit
GGCTGCATCACGGTCAAATTCAGATTTAGCCACATTCAAAGACTTCTTCA2850.102171045084318No Hit
CTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAA2840.1018125501892853No Hit
CTAGGTTCCATTGTTCAAGGAGCTTTTTAAGCTCTTCAACGGTAATAGTA2840.1018125501892853No Hit
GTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCT2830.10145405529425261No Hit
GGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAAC2820.10109556039921991No Hit
AACATGTTGTGCCAACCACCATAGAATTTGCTTGTTCCAATTACTACAGT2810.10073706550418722No Hit
GGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTATTATGTTCAA2800.10037857060915453No Hit
GTTGGAGTGTTAACAATGCAGTGGCAAGATAACAGTTGTTATCTGCCCAT2800.10037857060915453No Hit
ACAAAAACTGCCATATTGCAACAAAAGATTGCTGCATTCAGTTGAATCAC2790.10002007571412183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)