NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 576.3
Mean read quality 7.9
Median read length 165.0
Median read quality 10.9
Number of reads 65,377.0
Read length N50 15,914.0
STDEV read length 8,137.0
Total bases 37,678,013.0
Number, percentage and megabases of reads above quality cutoffs
>Q10 35630 (54.5%) 6.8Mb
>Q15 18574 (28.4%) 3.8Mb
>Q20 6034 (9.2%) 1.3Mb
>Q25 1522 (2.3%) 0.3Mb
>Q30 313 (0.5%) 0.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 43.5 (317)
2 40.4 (110)
3 40.4 (176)
4 39.7 (146)
5 39.2 (148)
Top 5 longest reads and their mean basecall quality score
1 728009 (5.9)
2 598559 (4.3)
3 550665 (4.1)
4 531690 (2.3)
5 526907 (5.4)

Summary statistics after filtering

General summary
Mean read length 576.3
Mean read quality 7.9
Median read length 165.0
Median read quality 10.9
Number of reads 65,376.0
Read length N50 15,914.0
STDEV read length 8,137.0
Total bases 37,677,999.0
Number, percentage and megabases of reads above quality cutoffs
>Q10 35629 (54.5%) 6.8Mb
>Q15 18573 (28.4%) 3.8Mb
>Q20 6033 (9.2%) 1.3Mb
>Q25 1521 (2.3%) 0.3Mb
>Q30 312 (0.5%) 0.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 43.5 (317)
2 40.4 (110)
3 40.4 (176)
4 39.7 (146)
5 39.2 (148)
Top 5 longest reads and their mean basecall quality score
1 728009 (5.9)
2 598559 (4.3)
3 550665 (4.1)
4 531690 (2.3)
5 526907 (5.4)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length