NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,362.3
Mean read quality 11.5
Median read length 559.0
Median read quality 12.3
Number of reads 301,346.0
Read length N50 3,075.0
STDEV read length 6,140.1
Total bases 410,529,960.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 244878 (81.3%) 346.3Mb
>Q7 213604 (70.9%) 322.5Mb
>Q10 179908 (59.7%) 292.2Mb
>Q12 155519 (51.6%) 262.0Mb
>Q15 101031 (33.5%) 183.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 36.1 (141)
2 35.9 (603)
3 35.9 (108)
4 35.3 (157)
5 35.1 (155)
Top 5 longest reads and their mean basecall quality score
1 923279 (4.6)
2 899654 (2.2)
3 882992 (2.7)
4 628307 (2.5)
5 589475 (4.2)

Summary statistics after filtering

General summary
Mean read length 1,362.3
Mean read quality 11.5
Median read length 559.0
Median read quality 12.3
Number of reads 301,345.0
Read length N50 3,075.0
STDEV read length 6,140.1
Total bases 410,529,950.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 244877 (81.3%) 346.3Mb
>Q7 213603 (70.9%) 322.5Mb
>Q10 179907 (59.7%) 292.2Mb
>Q12 155518 (51.6%) 262.0Mb
>Q15 101030 (33.5%) 183.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 36.1 (141)
2 35.9 (603)
3 35.9 (108)
4 35.3 (157)
5 35.1 (155)
Top 5 longest reads and their mean basecall quality score
1 923279 (4.6)
2 899654 (2.2)
3 882992 (2.7)
4 628307 (2.5)
5 589475 (4.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length