NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 238.4
Mean read quality 14.6
Median read length 203.0
Median read quality 14.2
Number of reads 125,019.0
Read length N50 268.0
STDEV read length 338.8
Total bases 29,808,208.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 124524 (99.6%) 29.8Mb
>Q7 123676 (98.9%) 29.6Mb
>Q10 118126 (94.5%) 28.8Mb
>Q12 99660 (79.7%) 24.9Mb
>Q15 49697 (39.8%) 12.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 37.4 (286)
2 37.4 (64)
3 36.9 (245)
4 36.5 (337)
5 36.4 (67)
Top 5 longest reads and their mean basecall quality score
1 85696 (8.4)
2 60744 (5.6)
3 16753 (5.0)
4 10740 (4.1)
5 7191 (14.0)

Summary statistics after filtering

General summary
Mean read length 238.4
Mean read quality 14.6
Median read length 203.0
Median read quality 14.2
Number of reads 125,016.0
Read length N50 268.0
STDEV read length 338.8
Total bases 29,808,166.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 124521 (99.6%) 29.8Mb
>Q7 123673 (98.9%) 29.6Mb
>Q10 118123 (94.5%) 28.8Mb
>Q12 99657 (79.7%) 24.9Mb
>Q15 49694 (39.8%) 12.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 37.4 (286)
2 37.4 (64)
3 36.9 (245)
4 36.5 (337)
5 36.4 (67)
Top 5 longest reads and their mean basecall quality score
1 85696 (8.4)
2 60744 (5.6)
3 16753 (5.0)
4 10740 (4.1)
5 7191 (14.0)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length