NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 420.1
Mean read quality 15.6
Median read length 381.0
Median read quality 15.1
Number of reads 1,379,889.0
Read length N50 439.0
STDEV read length 231.1
Total bases 579,724,008.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 1378775 (99.9%) 579.5Mb
>Q7 1376762 (99.8%) 579.0Mb
>Q10 1355667 (98.2%) 572.2Mb
>Q12 1201873 (87.1%) 511.4Mb
>Q15 698665 (50.6%) 295.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 40.3 (149)
2 40.1 (428)
3 39.7 (228)
4 39.7 (237)
5 39.6 (141)
Top 5 longest reads and their mean basecall quality score
1 33766 (4.0)
2 32763 (4.0)
3 18518 (16.4)
4 17345 (3.0)
5 8439 (16.2)

Summary statistics after filtering

General summary
Mean read length 420.1
Mean read quality 15.6
Median read length 381.0
Median read quality 15.1
Number of reads 1,379,883.0
Read length N50 439.0
STDEV read length 231.1
Total bases 579,723,944.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 1378769 (99.9%) 579.5Mb
>Q7 1376756 (99.8%) 579.0Mb
>Q10 1355661 (98.2%) 572.2Mb
>Q12 1201867 (87.1%) 511.4Mb
>Q15 698659 (50.6%) 295.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 40.3 (149)
2 40.1 (428)
3 39.7 (228)
4 39.7 (237)
5 39.6 (141)
Top 5 longest reads and their mean basecall quality score
1 33766 (4.0)
2 32763 (4.0)
3 18518 (16.4)
4 17345 (3.0)
5 8439 (16.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length