NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 537.9
Mean read quality 15.8
Median read length 438.0
Median read quality 15.4
Number of reads 1,221,711.0
Read length N50 638.0
STDEV read length 373.4
Total bases 657,204,933.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 1220882 (99.9%) 657.0Mb
>Q7 1219492 (99.8%) 656.6Mb
>Q10 1206736 (98.8%) 652.1Mb
>Q12 1099606 (90.0%) 604.5Mb
>Q15 667565 (54.6%) 386.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.1 (264)
2 40.2 (564)
3 39.8 (65)
4 39.6 (184)
5 38.8 (435)
Top 5 longest reads and their mean basecall quality score
1 33080 (6.0)
2 31371 (2.6)
3 16999 (2.8)
4 12807 (3.0)
5 12315 (19.1)

Summary statistics after filtering

General summary
Mean read length 537.9
Mean read quality 15.8
Median read length 438.0
Median read quality 15.4
Number of reads 1,221,706.0
Read length N50 638.0
STDEV read length 373.4
Total bases 657,204,898.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 1220877 (99.9%) 657.0Mb
>Q7 1219487 (99.8%) 656.6Mb
>Q10 1206731 (98.8%) 652.1Mb
>Q12 1099601 (90.0%) 604.5Mb
>Q15 667560 (54.6%) 386.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.1 (264)
2 40.2 (564)
3 39.8 (65)
4 39.6 (184)
5 38.8 (435)
Top 5 longest reads and their mean basecall quality score
1 33080 (6.0)
2 31371 (2.6)
3 16999 (2.8)
4 12807 (3.0)
5 12315 (19.1)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length