NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 470.9
Mean read quality 15.6
Median read length 405.0
Median read quality 15.2
Number of reads 1,338,218.0
Read length N50 517.0
STDEV read length 313.8
Total bases 630,205,582.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 1336889 (99.9%) 629.9Mb
>Q7 1334714 (99.7%) 629.4Mb
>Q10 1316065 (98.3%) 623.2Mb
>Q12 1178069 (88.0%) 566.6Mb
>Q15 701862 (52.4%) 348.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 43.9 (165)
2 41.6 (404)
3 40.5 (192)
4 40.4 (151)
5 40.3 (444)
Top 5 longest reads and their mean basecall quality score
1 103500 (4.5)
2 76797 (3.3)
3 33669 (9.1)
4 16947 (5.2)
5 16652 (8.1)

Summary statistics after filtering

General summary
Mean read length 470.9
Mean read quality 15.6
Median read length 405.0
Median read quality 15.2
Number of reads 1,338,214.0
Read length N50 517.0
STDEV read length 313.8
Total bases 630,205,538.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 1336885 (99.9%) 629.9Mb
>Q7 1334710 (99.7%) 629.4Mb
>Q10 1316061 (98.3%) 623.2Mb
>Q12 1178065 (88.0%) 566.6Mb
>Q15 701858 (52.4%) 348.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 43.9 (165)
2 41.6 (404)
3 40.5 (192)
4 40.4 (151)
5 40.3 (444)
Top 5 longest reads and their mean basecall quality score
1 103500 (4.5)
2 76797 (3.3)
3 33669 (9.1)
4 16947 (5.2)
5 16652 (8.1)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length