NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 441.6
Mean read quality 15.4
Median read length 392.0
Median read quality 14.9
Number of reads 1,116,385.0
Read length N50 474.0
STDEV read length 311.1
Total bases 492,962,811.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 1115612 (99.9%) 492.6Mb
>Q7 1114363 (99.8%) 492.1Mb
>Q10 1098620 (98.4%) 487.3Mb
>Q12 976202 (87.4%) 439.5Mb
>Q15 547983 (49.1%) 252.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 40.9 (187)
2 40.8 (284)
3 40.5 (69)
4 39.7 (28)
5 39.6 (107)
Top 5 longest reads and their mean basecall quality score
1 128999 (7.0)
2 95187 (4.3)
3 56145 (5.1)
4 37212 (4.9)
5 34931 (6.9)

Summary statistics after filtering

General summary
Mean read length 441.6
Mean read quality 15.4
Median read length 392.0
Median read quality 14.9
Number of reads 1,116,382.0
Read length N50 474.0
STDEV read length 311.1
Total bases 492,962,776.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 1115609 (99.9%) 492.6Mb
>Q7 1114360 (99.8%) 492.1Mb
>Q10 1098617 (98.4%) 487.3Mb
>Q12 976199 (87.4%) 439.5Mb
>Q15 547980 (49.1%) 252.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 40.9 (187)
2 40.8 (284)
3 40.5 (69)
4 39.7 (28)
5 39.6 (107)
Top 5 longest reads and their mean basecall quality score
1 128999 (7.0)
2 95187 (4.3)
3 56145 (5.1)
4 37212 (4.9)
5 34931 (6.9)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length