NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 452.2
Mean read quality 15.4
Median read length 346.0
Median read quality 15.0
Number of reads 230,865.0
Read length N50 571.0
STDEV read length 439.2
Total bases 104,398,663.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 230482 (99.8%) 104.3Mb
>Q7 229820 (99.5%) 104.2Mb
>Q10 226387 (98.1%) 103.6Mb
>Q12 206239 (89.3%) 96.6Mb
>Q15 116819 (50.6%) 56.8Mb
Top 5 highest mean basecall quality scores and their read lengths
1 39.7 (255)
2 37.1 (228)
3 37.0 (339)
4 37.0 (95)
5 36.9 (116)
Top 5 longest reads and their mean basecall quality score
1 110024 (4.9)
2 8673 (14.4)
3 7946 (14.0)
4 7662 (17.3)
5 7346 (18.2)

Summary statistics after filtering

General summary
Mean read length 452.2
Mean read quality 15.4
Median read length 346.0
Median read quality 15.0
Number of reads 230,859.0
Read length N50 571.0
STDEV read length 439.2
Total bases 104,398,595.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 230476 (99.8%) 104.3Mb
>Q7 229814 (99.5%) 104.2Mb
>Q10 226381 (98.1%) 103.6Mb
>Q12 206233 (89.3%) 96.6Mb
>Q15 116813 (50.6%) 56.8Mb
Top 5 highest mean basecall quality scores and their read lengths
1 39.7 (255)
2 37.1 (228)
3 37.0 (339)
4 37.0 (95)
5 36.9 (116)
Top 5 longest reads and their mean basecall quality score
1 110024 (4.9)
2 8673 (14.4)
3 7946 (14.0)
4 7662 (17.3)
5 7346 (18.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length